Rapid extraction of triglycerides from human adipose tissue with petroleum ether.

نویسندگان

  • H A Newman
  • E A Gordon
  • D W Heggen
  • M D Keller
چکیده

A rapid method has been developed for extracting neutral lipid from small samples of human adipose tissue, with a petroleum ether (“Skellysolve B”) as the extracting solvent. It is coupled with a simple method of transesterification, to convert the purified triglycerides to fatty acid methyl esters. Virtually no phospholipid is extracted by the petroleum ether. Analysis of the extracted lipid by gas-liquid chromatography yielded fatty acid distributions comparable to those obtained by chloroform-methanol extraction.

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عنوان ژورنال:
  • Clinical chemistry

دوره 18 3  شماره 

صفحات  -

تاریخ انتشار 1972